02026nam a22002057a 4500 D0001J000003 DOH 20210206133601.0 210206b ||||| |||| 00| 0 eng d Molecular characterization of hepatitis G virus (HGV) isolates from healthy adults and risk groups in the Philippines / Ja Ricaforte-Campos [and three others] Hepatitis G virus (HGV) infection has been detected in Filipinos through a prospective study of 1,088 blood samples. HGV RNA was found in sera of 6/516 (1.2 percent) healthy adults (volunteer blood donors), 11/138 (8.0 percent) chronic liver disease patients, 7/207 (3.4 percent) hemodialysis patients, and 14/227 (6.2 percent) multiply transfused patients using reversetranscription polymerase chain reaction (RT-PCR) with random hexamer primers and a set of PCR primers from the 5 untranslated region (5UTR) of the HGV genome. A total of 38/1,088 subjects were HGV RNA-positive The PCR products derived from the 5UTR of HGV RNA+ samples were sequenced to determine the genotypic variant of HGV in the Philippines. Pairwise alignment of sequences and phylogenetic tree construction revealed that among the five known HGV genotypic variants, the Philippine isolates are most closely related to the Asian type (III) Considering that HGV is a highly mutable organism, surveillance for new genotypes may be the only way to assess accurate asymptomatic infection rates. How much this virus evolves in the future may have an impact on its virulence, transmissibility and invasiveness. It is therefore important, from an evolutionary perspective, to continue and monitor evolution of HGV specifically with studies at the molecular level. (Author) In: Acta Medica Philippina, 2005 vol. 39 (1) pages: 13-21 GB virus C Hepatitis G Ricaforte-Campos, Jane D. Domingo, Ernesto O. Libranda-Ramirez, Bernadette DL Dalmacio, Leslie Mic, JA 2744 2744 0 0 nlm 0 0 DOHCL DOHCL ElectronicRes 2021-02-06 0 J000003 D0001J000003 2021-02-06 00:00:00 2021-02-06 JA