01965nam a22002057a 4500 D0001J000327 DOH 20210325140641.0 210325b ||||| |||| 00| 0 eng d Determination of sweeteners, benzoate and caffeine in carbonated non-alcoholic beverages by reversed-phase liquid chromatography / Jocelyn E. Balderrama, Carmina J. Parce A simple and rapid method for simultaneous determination of acesulfame-K, aspartame, saccharin, sodium benzoate, and caffeine in non-alcoholic beverages was validated using the Shimadzu LC system. The additives were separated on a Shimpack C18 column with a mobile phase composed of methanol-acetic acid-water (17+5+78) while the column over temperature was set at 40 C and the UV absorption measured at 254 nm wavelength. The retention time and detection limit for each additive were described. Measurement repeatability of the standards and their % recoveries from spiked samples ranged from 2.5% to 3.2% and from 97.5% to 110.2%, respectively Direct single step method of analysis was used to detect the levels of additives present in different brands of cola and non-cola carbonated beverages. All additives detected in the 26 samples analyzed were within prescribed levels. The diet soft drinks contained only one type of sweetener (aspartame). The instability of aspartame in these beverages were confirmed as the samples analyzed after marked dates had lower levels of aspartame and had notable loss in sweetness. Consumers were advised to take note of date markings on aspartame sweetened beverages and avoid those without them, especially the imported products.(Author) BFAD Laboratory Information Bulletin, 1996 2 (1) pages 11-16 Carbonated Beverages analysis Sweetening Agents Benzoates Caffeine Balderrama, Jocelyn E. Parce, Carmina J. JA 3778 3778 0 0 nlm 0 0 DOHCL DOHCL ElectronicRes 2021-03-25 0 J000327 D0001J000327 2021-03-25 00:00:00 2021-03-25 JA