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245	0	4	_aThe effect of probiotic (Lactobacilli) in experimentally-induced intestinal ischemia in mice / _cMa. Isabel F. Trapaga-Avancena , Pamela D. Cortez
520	3		_aBACKGROUND: Probiotics are defined as live microbial feed supplements, which beneficially affects the host by improving intestinal microbial balance. Their use for acute rotavirus diarrhea, antibiotic induced diarrhea and enterocolitis have been well elucidated. However, there is a paucity of studies regarding its role in the treatment of other intestinal disorders of graver magnitude such as those resulting from intestinal ischemia. OBJECTIVES: To determine the effects of probiotics on experimentally induced ischemia in mice specifically on histology of the areas of ischemia and to correlate clinical behavior after administration of treatment. SETTING AND SUBJECTS: A controlled laboratory study using ICR mice, weighing 27-33g, housed under standardized conditions in a research laboratory. METHODS: Intestinal ischemia was produced in ether anesthetized mice by forming a loop in the terminal 2 cm of the jejunum then ligating the area using silk suture. At the end of the ischemic period of thirty minutes, the suture was removed allowing reperfusion and the abdomen was closed. The mice were randomly divided into four groups, each group to receive either of the following: antibiotic alone, antibiotic plus lactobacilli, placebo (control), or lactobacilli alone. Clinical parameters were observed during the 4 day treatment period. The mice were then sacrificed. The area of ischemia was examined by a blinded pathologist and was graded according to severity of the findings. Results were then subjected to statistical analysis using the kruskal-wallis method. RESULTS: The treatment groups showed better outcome compared to the control according to clinical and histopathologic parameters. Those animals who received both antibiotic and lactobacilli showed the least desquamation and mucosal injury, least edema, less blunted villi, with the most number of inflammatory cells. CONCLUSION: Probiotic lactobacilli may be beneficial as an adjunct in the treatment of diseases caused by intestinal ischemia. Clinically and histopathologically those who received both the antibiotic and lactobacilli showed better outcome and better mucosal morphology respectively. Lactobacilli as a sole treatment for intestinal ischemia showed minimal therapeutic advantage.
580			_a Makati Medical Center Proceedings, 1999 (13) pages 78-83
650		2	_aLactobacillus _xdrug effects
650		2	_aIschemia _xpathology
650		2	_aMice
700	1		_aTrapaga-Avancena, Ma. Isabel F.
700	1		_aCortez, Pamela D.
942			_cJA
999			_c4738 _d4738