PCR patterns of the flagellin gene of local isolates of Salmonella typhi / (Record no. 2805)

MARC details
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fixed length control field 01905nam a22002057a 4500
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control field DOH
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20210211114700.0
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245 00 - TITLE STATEMENT
Title PCR patterns of the flagellin gene of local isolates of Salmonella typhi /
Statement of responsibility, etc. Ann Florence Victoriano [and two others]
520 3# - SUMMARY, ETC.
Summary, etc. Typhoid fever is a systemic disease caused by Salmonella typhi. It is a worldwide public health problem especially seen in developing countries with a disease burden of about 22 million cases per year. Because proper sanitation and hygiene practices are difficult to achieve and problems of drug resistance have emerged, vaccination is now being considered as an essential tool in the prevention and control of the disease. The present study is a preliminary undertaking in the development of an edible vaccine based on the flagellar antigen of the organism. Specifically, the study determined the similarity of the vaccine candidate with some of the local isolates. Using primers based on the vaccine candidate which spanned the 3' end of the hypervariable region VI up to the end of the flagellin gene, a 470 bp fragment was amplified in the 19 local isolates. Results showed that the different isolates have a high measure of similarity with the candidate strain. In spite of the presence of the hypervariable region VI of S. typhi flagellin gene, the lengths of the fragments are rather conserved among the isolates tested. The observations, even with the limited sample size, demonstrated that the candidate flagellin gene can be appropriately used for vaccine development.
580 ## - LINKING ENTRY COMPLEXITY NOTE
Linking entry complexity note In: Acta Medica Philippina, 2006 vol. 40 (1) pages: 37-41
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Typhoid Fever
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Salmonella typhi
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Flagellin
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Victoriano, Ann Florence
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sarol, Lilen C.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Gloriani-Barzaga, Nina
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journal Article
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-- 2805
Holdings
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    National Library of Medicine     DOH Central Library DOH Central Library Electronic Resource Section 02/11/2021   J000021 D0001J000021 02/11/2021 02/11/2021 Journal Article