Improved screening efficiency for phenylketonuria using a modified bacterial inhibition assay protocol- Autoclaving the bloodspot / (Record no. 3115)

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Title Improved screening efficiency for phenylketonuria using a modified bacterial inhibition assay protocol- Autoclaving the bloodspot /
Statement of responsibility, etc. Acta Medica Philippina, 2009 vol 43 (2) pages: 29-31
520 3# - SUMMARY, ETC.
Summary, etc. The Guthrie bacterial inhibition assay (BIA) tests for elevated phenylalanine (PHE) by measuring B. subtilis growth zone density in an agar medium. Dried blood spots with elevated PHE on initial BIA screening undergo repeat BIA testing and thin-layer chromatography (TLC). Specimens with elevated PHE by TLC or BIA on second-tier testing require recall. To streamline PKU screening and reduce the recall rate, we tested a modified BIA protocol incorporating autoclaving of dried blood spots. Autoclaving improves growth zone appearance and has been previously reported to reduce the number of specimen requiring repeat testing. From June to October 2006, dried blood spot samples with initially elevated PHE were autoclaved at 110°C for 5 min, then retested by BIA. Samples with still-elevated PHE were analyzed by TLC. 1078 of 37,268 samples (2.89%) had initially elevated PHE. After autoclaving, 1036 no longer exhibited elevated PHE decreasing to 42 (0.11%) the number requiring TLC. By comparison, the unmodified algorithm resulted in 3.14% of samples received from July - December 2006 requiring both repeat BIA and TLC testing. We have since modified our PKU screening algorithm to require repeat BIA testing from autoclaved samples prior to TLC analysis. This translates to a significant reduction in time and resources for second-tier testing and follow-up, and prevents stress for the parents of a newborn who would have been recalled unnecessarily.
580 ## - LINKING ENTRY COMPLEXITY NOTE
Linking entry complexity note In: Acta Medica Philippina, 2009 vol 43 (2) pages: 29-31
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Phenylketonurias
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Carrillo, Maria Constancia O.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Tirona, Joy
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Capistrano-Estrada, Sylvia
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name David-Padilla, Carmencita
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Journal Article
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-- 3115
Holdings
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    National Library of Medicine     DOH Central Library DOH Central Library Electronic Resource Section 02/27/2021   J000107 D0001J000107 02/27/2021 02/27/2021 Journal Article