Determination of chloramphenicol sodium succinate in human plasma by reversed-phase liquid chromatography / (Record no. 3772)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01886nam a22002057a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DOH |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20210325104917.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210324b ||||| |||| 00| 0 eng d |
| 245 00 - TITLE STATEMENT | |
| Title | Determination of chloramphenicol sodium succinate in human plasma by reversed-phase liquid chromatography / |
| Statement of responsibility, etc. | Josephine K. Sarau [and two others] |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A reversed-phase HPLC method for determination of chloramphenicol sodium succinate in blood plasma of Filipino children has been developed and validated. The method involves the addition of internal standard, precipitation and liquid-liquid extraction of the spiked or control samples, evaporation under nitrogen gas, processed through a reversed-phase HPLC column, followed by UV detection. The extracted sample was analyzed through a Lichrosorb RP-18 (4.0 x 125 mm) column, using methanol:water (35:65) as mobile phase, and detected by UV absorbance at N=281 nm. The retention time was 10 min. for chloramphenicol sodium succinate and 14 min. for internal standard. The assay was linear from 0.05 to 11 ug/ml (r = 0.99833), with inter-precision (RSD) of 6.173% (n=6), 3.656% (n=6), and 6.002% (n=6), for high (3.48 ug/ml), medium (1.08 ug/ml), and low (0.58% ug/ml), spiked or control samples, respectively. Percentage recovery is greater than 90%. With the results generated, the method was used in the pharmacokinetic study of chloramphenicol in children with severe pneumonia in a collaborative work with Clinical Pharmacology Section headed by Dr. Co, University of Santo Tomas College of Medicine and Surgery. (Author) |
| 580 ## - LINKING ENTRY COMPLEXITY NOTE | |
| Linking entry complexity note | BFAD Laboratory Information Bulletin, 1995 1 (1) pages 25-34 |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Succinates |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plasma |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chloramphenicol |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chromatography, Reverse-Phase |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sarau, Josephine K. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Elveña, Elisea B. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Co, Benjamin G. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| 999 ## - | |
| -- | 3772 |
| -- | 3772 |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| National Library of Medicine | DOH Central Library | DOH Central Library | Electronic Resource Section | 03/24/2021 | J000321 | D0001J000321 | 03/24/2021 | 03/24/2021 | Journal Article |