Fluid replacement by the Intravenous route have become an integral part of patient care but outbreaks of septicemia caused by contaminated infusion products, have dramatically focused our attention on the risks brought about by IV fluids. Studies have shown that most infusion related septicemia are caused by the cannula used for IV access but the fluid administered can be the culprit. This study aims to find out if there will be bacterial growth in 50 bottles 250 cc Dextrose 5% water after puncture and storage in the NICU setting. Results revealed bacterial growth in 10% (5 bottles) at immediate puncture, 16% (8 bottles) after 12 hours of storage and 24% (12 bottles) after 24 hours of storage. The isolates were predominantly Coagulase Negative Staphylococci, then Pseudomonas sp. and Acinetobacter sp. These microorganisms are the same ones that cause clinical infection in the NICU. This may point to a deviation from the aseptic techniques by the NICU staff including the storing of IV solutions up to 24 hrs. Recommendations include strict reinforcement on the employment of aseptic techniques on the handling and preparation of Intravenous infusions. (Author)

Makati Medical Center Proceedings, 1999 (13) pages 67-70